DNA Extraction and Purification
Biochain Institute Inc SeqPure PCR Purification Kit (500 ml), 500 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Macherey-Nagel NucleoSpin Gel and PCR Clean-up, 10 preps
NucleoSpin Gel and PCR Clean-up (10) 10 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers
New England Biolabs, Inc. NEBNext® Ultra™ II Q5® Master Mix – 500 reactions
The NEBNext Ultra II Q5 Master Mix is the most recent formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries. This new formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´- 5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 280-fold higher than that of Taq DNA Polymerase), resulting in ultra-low error rates. The NEBNext Ultra II Q5 Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.
ATCC Quantitative Genomic; RNA from Influenza; B virus; Strain: B/Lee/40
Quantitative Genomic RNA from Influenza B virus strain B/Lee/40 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Omega Biotek Inc Plasmid DNA Mini Kit I, (V-spin), 50 preps
Isolate up to 25 ug plasmid DNA from 1-5 mL bacterial cultures using mini spin columns.
New England Biolabs, Inc. NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® – 96 reactions
The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification.
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix
- Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
- Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
- Use with DNA in standard buffers (TE, Tris-HCl) and water
Quantitative Genomic RNA from Influenza A virus H1N1 strain A/California/07/2009 (H1N1)pdm09 (ATCC VR-1894DQ™)
Quantitative Genomic RNA from Influenza A virus H1N1 strain A/California/07/2009 (H1N1)pdm09 (ATCC VR-1894DQ™) is a molecular standard used for qPCR assay development, validation, and viral load quantification. This product is recommended for respiratory disease research and vaccine-related assay workflows.
Zymo Research Corporation ZYMOPURE-96 PLASMID MINIPREP
ZymoPURE-96 Plasmid Miniprep Kit (2 x 96 Preps.)
Zymo Research Corporation Micrococcal Nuclease (10 U /100 μl)
Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3-phosphates.
New England Biolabs, Inc. Monarch® gDNA Purification Columns - 100 columns
The Monarch gDNA Purification Columns are a component of the Monarch Genomic DNA Purification Kit and can be used to purify up to 30 ug genomic DNA from a variety of sample types. They are available for purchase separately for your convenience and supplied with Monarch Collection Tubes II.
- A component of the Monarch Genomic DNA Purification Kit
- Can be used to purify up to 30 ug genomic DNA from a variety of sample types
- Contains 100 columns and 100 collection tubes
- Compatible with Monarch Collection Tubes II
- Monarch kit components are also available separately for added convenience
New England Biolabs, Inc. Exo-CIP Rapid PCR Cleanup Kit – 400 reactions
The Exo-CIP Rapid PCR Cleanup Kit contains optimized formulations of thermolabile Exonuclease I and thermolabile Calf Intestinal Phosphatase (CIP) and is used to rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification. Degradation occurs in only 4 minutes at 37C, and is immediately followed by rapid inactivation of the enzymes by heating for 1 minute at 80C. In just 5 minutes, the PCR product is ready for downstream analysis, such as Sanger Sequencing, SNP detection, or library preparation for NGS. The Exo-CIP Rapid PCR Cleanup Kit is compatible with all commonly-used PCR buffers.
New England Biolabs, Inc. Monarch® Spin gDNA Extraction Kit - 150 preps
The Monarch Spin gDNA Extraction Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Monarch products use significantly less plastic in columns and kits compared to leading suppliers. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms
New England Biolabs, Inc. Monarch® gDNA Elution Buffer - 34 ml
Monarch gDNA Elution Buffer is supplied in 10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA and is suitable for long term storage of gDNA. Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution. This buffer is a component of the Monarch Genomic DNA Purification Kit.
- A component of the Monarch Genomic DNA Purification Kit
- 10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA
- Suitable for long term storage of gDNA
- Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution
- Monarch kit components are also available separately for added convenience